Factors to Consider When Extracting DNA Molecule
The most important component in the human’s blood is the DNA molecule and is the first thing looked into incase of any problem. The component is formed from the fusion of the cells of the two parents who are responsible for the birth of the baby. The identification mark for any wanted person in the current society is the use of the DNA and it can be traced from the fingerprints of a person. The cell normally a lot of components and to get each one of them, separation has to be made. The whole process is a bit strenuous and is very tough thus takes quite some time to come up with the correct thing.
All the tools used in the extraction have to be purely clean and even when doing the extraction to avoid minor mistakes that might cost the whole activity. Methods of extracting it are three and the most suitable one can be used, the incubation method has to be done overnight, the reagent one which is newly installed and there is the protocol type which is the easiest method completed in just some few minutes. There are situations that can only restrict the use of a certain method depending with the current conditions and the people involved will have to follow them.
In the extraction, there are some factors that help identify the DNA from the rest of the components. The negative charges of the DNA molecule make it possible for them to be identified from the rest of the molecules. The DNA molecule is made up of the many chromosomes that are coiled and this makes up the structure of them and since they are negatively charged and the proteins are usually positively charged which makes them attract to each other.
The steps in the DNA extraction are three and have to be followed systematically to acquire the exact results. The cells have to be broken open first for all the components in it to disintegrate and get separated to enable one have a starting point. It cannot be possible to just start working on the whole cell molecule yet it has very many components with it. The best way to open the cell membrane without problems is using heat or detergents to smoothen it.
DNA features play the role of proper separation without confusion. It is necessary to put into consideration some important factors have to be considered to avoid confusion. After the molecules have been extracted, they have to be isolated for use.